michael kors black Friday "Without a renovated or modernized stadium, Miami is not going to get Super Bowls, and I don't want us to become another San Diego," Ross said. "People love going to San Diego. They didn't do anything to the stadium, and they haven't had a Super Bowl there in what, 15 or 20 years even though they love the experience there.".The requirement for liganddependent processing for Notch activity was verified using mutants disabled for interactions with ligand. The recombinant Notch1LNG, which lacks the epidermal growth factor (EGF)like repeats in its extracellular domain,20 did not block death (Figure 2b), whereas a construct corrected for this defect (Notch1LNG CCSS) imparted protection from death (Figure 2b). As expected, only Notch1LNG CCSS activated the Hes1 promoter (a readout of transcriptional activity) in reporter assays (Figure 2d).
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