ugg black friday sale 2013 Cell culture and retroviral transduction of Hut78 cellsHut78 cells were cultured in Rosewell Park Memorial Institute 1640 medium plus 10% FCS and 104 M 2mercaptoethanol (Sigma, Saint Louis, MO, USA). The cells were resuspended at 1 105 cells/ml in the viruscontaining medium diluted 1:2 in DMEM containing 15% FCS and protamine sulfate (5 g/ml) for 4 h. The cells were then incubated at 37 for a further 48 h and fluoresceinactivated cell sorter (FACS) sorting was performed to purify green fluorescent protein (GFP)+ cells from transduced cells."I think anything that's happened in Ohio, certainly Gov. Kasich has been helpful to encouraging business in Ohio. And it just shows, whether it's Ohio or New Jersey or Indiana, when you have Republican governors who encourages business, things are better," Schriefer said in a conference call, adding that Romney would be more like Republican governors who are